Journal: Arthritis Research & Therapy

Article Title: Circ-CAMTA1 regulated by Ca 2+ influx inhibited pyruvate carboxylase activity and modulate T cell function in patients with systemic lupus erythematosus

doi: 10.1186/s13075-024-03422-6

Figure Lengend Snippet: The expression levels of circ-CAMTA1, circ-ASH1L, and circ-ASAP1 in Jurkat cells after stimulation. Jurkat cells were stimulated with PMA 20 ng/mL + Ionomycin (Iono) 500 ng/mL for 24 h. ( A ) The expression levels of circ-CAMTA. ( B ) The expression levels of circ-ASH1L. ( C ) The expression levels of circ-ASAP1. Jurkat cells were stimulated with 1000 IU/mL recombinant human interferon-alpha (IFN-α) for 72 h. ( D ) The expression levels of circ-CAMTA1. ( E ) The expression levels of circ-ASH1L. ( F ) The expression levels of circ-ASAP1. Data was presented as mean ± SD. (ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: To analyzed circRNA expression levels in Jurkat cells after stimulation, cells were treated with phorbol 12-myristate 13-acetate (PMA; 20 ng/mL; Sigma-Aldrich) and ionomycin (500 ng/mL; Sigma-Aldrich, ) for 24 h. For analysis of circRNA expression in response to interferon-alpha (IFN-α), Jurkat cells were treated with 1000 IU/mL recombinant human IFN-α for 72 h. The IFN-alpha we used was purchased from Proteintech (Rosemont, IL, USA) and was produced in HEK293.

Techniques: Expressing, Recombinant

Journal: medRxiv

Article Title: Polymorphism in IFNAR contributes to glucocorticoid response and outcome in ARDS and COVID-19

doi: 10.1101/2022.03.10.22272123

Figure Lengend Snippet: (A ) STAT1 expression in the lung after 4-day culture in the presence of IFN beta with or without hydrocortisone (HC). ( B) pSTAT1 expression in the same specimens as in A. ( C) Example photomicrographs showing higher STAT2 expression in a TT patient than in a CT patient and the effect of HC on its nuclear translocation. Most STAT2 remains in the cytoplasm of the CT patients, whereas nuclear expression is prominent in the TT patient. Indicated insets are shown in the bottom row. Arrows. ( D ) Combined results of all patients noting that two CT samples are excluded in the data as the patients were already under glucocorticoid treatment at the time of sample acquisition. Ns, not significant; *P<0.05; **P<0.01; and ***P<0.001

Article Snippet: The first stage antibodies were anti-alpha chain of the IFN alpha/beta receptor (St John’s Laboratory STJ112765) that was used 1:2000 and 1:5000 and anti-Stat1 (1:400, 9175S), anti-pStat1(1:100, 9167S) and anti-Stat2 (1:200, 72604S) all from Cell Signalling.

Techniques: Expressing, Translocation Assay